Objective: To design and establish UPLC fingerprint on several varieties of Pueraria from Sichuan

and provide basis for its medicinal materials identification and quality control. Method: The UPLC fingerprint was performed on Agilent Zorbax SB-C18 (2.1 mm×5 mm

1.8 μm)

with acetonitrile -0.1% acetic acid aqueous solution as mobile phase; the column temperature was 30℃; the flow rate was 0.4 mL·min-1 and injection volume was 0.5 μL; the detection wavelength was set at 250 nm. In addition

similarity analysis and cluster analysis were conducted. Result: The UPLC fingerprint of P. lobata

P. thomsonii and P. omeiensis were established

and respectively confirmed 29

18 and 16 peaks. Seven peaks in P. lobata and six peaks in P. thomsonii and P. omeiensis were identified by comparing the retention time of reference substance and chromatographic peak. The main differences between three varieties were peaks 2

13

14

16

23

25

26 and 29

which were not detected in P. thomsonii and P. omeiensis; the main differences between P. thomsonii and P. omeiensis were peaks 6 and 14

which were not detected in P. omeiensis. The correlation results obtained in clustering analysis were close to similarity analysis results. Conclusion: UPLC fingerprint technology is fast and its result is reliable and accurate

so it can be used as a reliable method for study on this kind of medicine fingerprint.