Objective: To establish the HPLC fingerprints of different polarity fractions from Henan Forsythia Fructus

compare their chemical compositions between the different polarity fractions

and provide a reference for comprehensive evaluation of the quality of Henan Forsythia Fructus. Method: Henan Forsythia Fructus was extracted by 75% ethanol to obtain the total extract

and then it was extracted by petroleum ether

ethyl acetate

chloroform

n-butanol

and water respectively; concentrated to extractum

and dried under reduced pressure to obtain powder samples. The samples were analyzed by HPLC fingerprints

and the similarity evaluation software was used for data analysis. Result: Eighteen common peaks were determined in HPLC fingerprints of petroleum ether fraction

and 3 peaks of them were identified; 25 common peaks were determined in HPLC fingerprints of chloroform extract fraction

and 4 peaks of them were identified; 20 common peaks were determined in the HPLC fingerprints of ethyl acetate fraction

and 3 peaks of them were identified; 17 common peaks were determined in HPLC fingerprint of n-butyl alcohol fraction

and 3 peaks of them were identified; 17 common peaks were determined in HPLC fingerprints of water extract

and 2 peaks of them were identified. The fingerprint similarity of the same polarity fractions of Forsythia Fructus was high

with significant differences in chemical compositions between different polarity fractions. Conclusion: The experimental method is accurate

reliable

and highly repeatable

and can fully reflect the distribution of the chemical composition of Forsythia Fructus

providing a reference for the comprehensive quality evaluation of Forsythia Fructus decoction pieces.