Objective: To establish a method of UPLC-Q-TOF/MS for determination of tumulosic acid and dehydro-tumulosic acid in rat plasma after oral administration of Poria triterpenoids extract powder and soft capsules content

differences of pharmacokinetics of tumulosic acid and dehydro-tumulosic acid in two forms were compared. Method: Liquid-liquid extraction was adopted to extract plasma samples with ethyl acetate

taking glycyrrhetic acid as internal standard

good chromatographic separation was achieved with the mobile phase consisting of 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water for gradient elution.Mass conditions were as following:positive sensitive mode

capillary voltage of 3 kV

ion source temperature of 120℃

desolvent temperature of 500℃ and desolvation gas flow of 800 L·h-1. Result: Cmax and AUC0-t of tumulosic acid and dehydro-tumulosic acid in soft capsules content were greater than those of powder.Tmax of tumulosic acid in soft capsules content was longer than that of the powder

but Tmax of dehydro-tumulosic acid was lower than that of powder. Conclusion: These results indicate that vegetable oil can promote the absorption of tumulosic acid and dehydro-tumulosic acid.It is probably that the different form of Poria triterpenoids extract lead to the change of absorption rate due to the different chemical structure of tumulosic acid and dehydro-tumulosic acid.