Objective: To analyze the compositions of Chinese herbal prescription Jiuwei Tongqiao decoction and its pharmacodynamic in vitro

explore its poaaile mechanism and provide experimental basis for its effectiveness. Method: Jiuwei Tongqiao decoction was separated into seven prescriptions based on the principles of Jun

Chen

Zuo

Shi. After alcohol extract enrichment

U251 cells of anthropogenic brain glioma were used inmethylthiazolyldiphenyl tetrazolium bromide (MTT) assay to determine the effect of different prescriptions on cell survival. Flow cytometry instrument was used to compare the effect of optimal prescription Ⅳ (10

4

1 g·L-1) and whole prescription on cells apoptosis. Western blot was used to detect apoptosis-related protein B-cell lymphoma-2 (Bcl-2) and p53 expression differences between the optimal prescription Ⅳ and the whole prescription. Alcohol extraction of the optimal prescription Ⅳ was concentrated with petroleum ether

chloroform and ethyl acetate

and then MTT was applied to determine the effect of various extraction parts on cell survival. Result: Prescription Ⅳ had the most obvious inhibition effect on U251 cells in a concentration-dependent manner. The apoptosis ratesinprescription Ⅳ treatment group (10

4

1 g·L-1)and whole prescription were all significantly higher than those in blank group after 24 h (P<0.01). The Bcl-2 protein expression levels of prescription Ⅳ group and the whole prescription group were significantly lower than those in blank group (P<0.01)

and p53 protein expression levels were significantly higher(P<0.01). The directive ingredients in part Ⅲ of prescription Ⅳ were more comprehensive after gradient extraction. Conclusion: Prescription Ⅳ is the optimal prescription

and part Ⅲ is the most effective part with the anti-proliferative effect on U251 glioma cells.