药效学结合正交试验优选参连颗粒的水提工艺

王敏; 杜守颖; 田志浩; 白洁; 李鹏跃; 张林; 黎丹奇; 万洁

doi:10.13422/j.cnki.syfjx.2017080027

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药效学结合正交试验优选参连颗粒的水提工艺

药剂与炮制 | 更新时间：2024-01-04

- 药效学结合正交试验优选参连颗粒的水提工艺
- Optimization of Water Extraction Process of Shenlian Granules by Orthogonal Test and Pharmacodynamics
- 中国实验方剂学杂志 2017年23卷第8期页码：27-31
- 作者机构：
- 作者简介：
- 基金信息：
  
  北京市科技计划项目（Z141100002214005）
- DOI：10.13422/j.cnki.syfjx.2017080027
  中图分类号： R283.6
- 纸质出版日期：2017
- 稿件说明：
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王敏, 杜守颖, 田志浩, 等. 药效学结合正交试验优选参连颗粒的水提工艺[J]. 中国实验方剂学杂志, 2017,23(8):27-31.

WANG Min, DU Shou-ying, TIAN Zhi-hao, et al. Optimization of Water Extraction Process of Shenlian Granules by Orthogonal Test and Pharmacodynamics[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(8): 27-31.
王敏, 杜守颖, 田志浩, 等. 药效学结合正交试验优选参连颗粒的水提工艺[J]. 中国实验方剂学杂志, 2017,23(8):27-31. DOI： 10.13422/j.cnki.syfjx.2017080027.

WANG Min, DU Shou-ying, TIAN Zhi-hao, et al. Optimization of Water Extraction Process of Shenlian Granules by Orthogonal Test and Pharmacodynamics[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(8): 27-31. DOI： 10.13422/j.cnki.syfjx.2017080027.

摘要

目的：多指标综合评分法优选参连方的水提工艺，为参连颗粒的生产提供工艺参数。方法：将大鼠随机分为空白组，提取物Ⅰ组（水提），提取物Ⅱ组（水提醇沉），提取物Ⅲ组（党参等水提醇沉，丹参等醇提），提取物Ⅳ组（党参等水提，丹参等醇提），在以大鼠心肌缺血再灌注后室性早搏、室性心动过速和室性颤动的总时间为指标考察不同提取工艺路线的基础上，采用HPLC测定芍药苷、甘草苷和甘草酸的含量，流动相乙腈（A）-0.1%磷酸水溶液（B）梯度洗脱（0~18 min，86% B；18~30 min，86%~72% B；30~40 min，72%~65% B；40~50 min，65%~60% B；50~55 min，60%~40% B；55~60 min，40%~86% B；60~65 min，86% B），流速1.0 mL·min-1，芍药苷检测波长230 nm，甘草苷和甘草酸检测波长237 nm。通过正交试验考察溶剂用量、提取时间、提取次数对3种成分含量的影响，利用综合评分法优选提取工艺并进行验证试验。结果：最佳提取工艺为提取3次，分别加10，8，8倍量水提取1.5 h；芍药苷、甘草苷、甘草酸提取量分别为22.88，15.17，13.54 mg·g-1。结论：优选的提取工艺稳定可行，可为参连颗粒的生产提供指导。

Abstract

Objective: To optimize the water extraction process of Shenlian formula. Method: Rats were randomized into control group

extract Ⅰ group (water extraction)

extract Ⅱ group (water extraction and ethanol precipitation)

extract Ⅲ group (water extraction and ethanol precipitation of some herbs

ethanol extraction of others)

extract Ⅳ group (water extraction of some herbs

ethanol extraction of others)

and the numbers of premature ventricular contraction

the total time of ventricular tachycardia and ventricular fibrillation were used to evaluate the arrhythmia induced by myocardial ischemia reperfusion arrhythmia.Orthogonal design was used to optimize the process conditions by taking solvent volume

extraction time and times as factors after investigating pharmacodynamics index of different extraction process routes.Paeoniflorin

liquiritin and glycyrrhizic acid

as examining parameters

were evaluated by HPLC with a mobile phase of acetonitrile-0.1% phosphoric acid solution for gradient elution. Result: Optimal extraction process was as follows:extracted thrice with 10

8 times the amount of water

decocted for 1.5 h per time.Extracting amounts of paeoniflorin

liquiritin and glycyrrhizic acid were 22.88

15.17

13.54 mg·g-1. Conclusion: This optimized extraction process is stable and feasible

which can provide reference value for the actual production of Shenlian granules.

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