Objective: To develop a certified reference material (CRM) of cinnamaldehyde according to The Directives for the Work of Reference Materials (3):Reference Material-General and Statistical Principles for Certification (GB/T 15000.3-2008) based on the CRM project approved by Standardization Administration of the People's Republic of China (SAC). Method: Cinnamaldehyde was obtained by means of extraction

NaHSO3 additive reaction

and preparative high performance liquid chromatography from the dried Cinnamomi Cortex. The structure of cinnamaldehyde was confirmed by element analysis

UV

IR

HRMS

NMR. Thin layer chromatography was carried out for the identification. The sample of cinnamaldehyde was divided into 200 bottles with 20 mg per bottle. The homogeneity test

stability test

and quantitative analysis were carried out by high performance liquid chromatography with an ultra violet detector. Result: The homogeneity of CRM of cinnamaldehyde was excellent

and the storage period for cinnamaldehyde was 24 months at 0-8℃. The certified purity value of the CRM of cinnamaldehyde was 98.41% with an expand uncertainty of 0.06%. This CRM corresponded with the technical requirement of a series of GB/T 15000.3 guides about the CRM and was checked and accepted by SAC. Conclusion: The national CRM of cinnamaldehyde was developed for the first time. This CRM is intended for use in the method validation

testing

and quality control regarding cinnamaldehyde.