Objective: To establish the HPLC fingerprint of Moringa oleifera leaves from different origins for identification and quality control

and promote the development of the modernization process of M. oleifera leaves. Method: HPLC was performed on the column of Diamonsil Plus C18 with acetonitrile (A)-0.05% phosphoric acid solution (B) as the mobile phase (0-70 min

5%-30%A; 70-75 min

30%-100%A) at a flow rate of 1.0 mL·min-1. The detective wavelength was 230 nm and the column temperature was 30℃. The fingerprints of 12 batches of M. oleifera leaves from 5 different origins were compared for similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2004 A) and the cluster analysis through SPSS 21.0. Result: The common mode for M. oleifera leaves fingerprints was established

and 14 common fingerprint peaks were identified; among them

three component peaks were gallic acid

rutin and quercetin which were the effective components; the similarity was greater than 0.90 in 9 batches of the 12 batches of M. oleifera leaves

indicating that propolis fingerprints had both similarities and differences either from different main producing areas or different fields within the same producing area. Conclusion: This method is reproducible

precise and steady and it can be used for origin identification and quality control of M. oleifera leaves.