Objective: To establish a multifactor composite model with cerebral infarction with Qi-deficiency and blood-stasis syndrome in rats. Method: Rats were randomly divided into 5 groups

namely sham operation group

multiple cerebral infarction group

multiple cerebral infarction combined with exhaustive swimming group

multiple cerebral infarction combined with sleep deprivation group

multiple cerebral infarction combined with both exhaustive swimming and sleep deprivation group and sham group. Rats of the three syndromes combined model groups were provided with the corresponding factors (sleep deprivation and exhaustive swimming) for 10 days. Then

the rats received multiple cerebral infarction surgery. After the surgery

sleep deprivation and exhaustive swimming were continuously implemented for another 10 days. The rats of multiple cerebral infarction group only received multiple cerebral infarction surgery. At the 11th day after the surgery

general status

body weight

grasping strength of fore limb

pulse

tongue picture

hemorheology and four items of blood coagulation were observed and assayed. Result: Body weight

grasping strength of fore limb

pulse intensity

and RGB value of tongue surface were decreased

while the whole blood viscosity increased

and prothrombin time(PT) and activated partial thromboplastin time(APTT) shortened in multiple cerebral infarction combined with sleep deprivation groups compared with sham operation group (P < 0.05

P < 0.01)

with more obvious changes in indexes than other groups. Conclusion: Through comprehensive analysis of traditional Chinese medicine syndromes (weight

grasping strength of fore limb

tongue

pulse) and western medicine biochemical indexes

the method of multiple cerebral infarction combined with sleep deprivation is superior to the other three modeling methods

and more suitable to establish a rat model with cerebral infarction with Qi-deficiency and blood-stasis syndrome.