Objective: To investigate the effect of total flavonoids from Milledttia Speciosa Radix (TFRMS) and Millettia Championi Radix(TFRMC) on acute lung injury (ALI) in mice models and observe the differences in anti-inflammatory effect between TFRMS and TFRMC. Method: ALI mice models were induced by lipopolysaccharide (LPS)

and then the anti-inflammation effect of TFRMS and TFRMC was observed in ALI mice models. The numbers of white blood cell (WBC) and the content of protein were determined in Bronchoalveolar lavage fluid (BALF). Then the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the lung tissues were detected by enzymelinked immunosorbent assay(ELISA)

the expression levels of nuclear factor-κB (NF-κB) p65 in lung tissues were determined by immunohistochemistry

the mRNA expression levels of IL-6 and TNF-α were detected by Real-time PCR method

and the pathological changes of lung tissues were observed by hematoxylin and eosin(HE) staining. Result: As compared with normal group

the count of WBC

protein content

levels of IL-6 and TNF-α were significantly increased in model group (P<0.01)

with inflammatory changes in lung tissues. As compared with the model group

the count of WBC and content of protein were decreased in BALF of TFRMS and TFRMC groups (P < 0.05

P < 0.01); the NF-κB p65 protein expression in lung tissues was significantly decreased (P<0.01); IL-6 and TNF-α mRNA expression levels and contents in the lung tissue were decreased (P < 0.05

P < 0.01). From the perspective of histopathology

various dose groups of TFRMS and TFRMC can reduce the acute lung injury induced by LPS. Conclusion: Both TFRMS and TFRMC had significant similar anti-inflammation effect

and their mechanism may be associated with interfering the NF-κB pathway

reducing the mRNA expression levels of IL-6 and TNF-α

and inhibiting IL-6 and TNF-α inflammatory mediators.