Objective: To investigate the anti-allergic asthma constituents in Tadehagi triquetrum. Method: The fractions were enriched from T. triquerum by using medium or high pressure liquid chromatography; such active fractions as G protein-coupled receptor-35 (GPR35) agonists were screened out by using label-free dynamic mass redistribution assays; HPLC-TOF-MS was used to analyze the main components in active fractions. Result: The 95% ethanol extracts of leaves of T. triquerum were extracted with n-heptane-70% methanol; 70% methanol extraction layer was eluted with 5%

70% and 95% methanol through C18 medium-pressure preparation column; among them

the part eluted with 70% methanol was eluted with methanol-water through XAmide high-pressure preparative chromatography column

and separated into 10 components based on chromatographic peaks. The 10 fractions were enriched from T. triquerum; fraction 4

5

6

7

9 and 10 exhibited strong activities against GPR35 receptor

suggesting the above components are anti-allergic asthma constituents of T. triquerum. HPLC-TOF-MS was used to analyze the chemical constituents of the above components and identify 12 constituents

namely ursonic acid

catechinic acid

kaempferol-3-O-α-rhamanopyranosyl(1→6)-β-glucoside

quercetin-3-O-α-rhamanopyranosyl(1→6)-β-glucoside/quercetin-3-O-α-rhamanopyranosyl(1→6)-β-galactoside

phloroglucinol-3-O-β-galactoside

triquetrumone E

triquetrumone F

kaempferol

daucosterol and apigenin. Conclusion: Fraction 4

5

6

7

9 and 10 are active fractions

and the compounds identified from active fractions might be anti-allergic asthma constituents.