Objective: To investigate the chemical constituents of Tripterospermum cordatum. Method: The herb of T. cordatum was crushed and then respectively extracted with 90% and 60% ethanol

and the extracts were combined and concentrated under reduced pressure. The residues were dispersed in water and respectively extracted with petroleum ether

ethyl acetate and butanol to sequentially get petroleum ether

ethyl acetate and butanol extracts. Ethyl acetate and butanol extracts were repeatedly separated and purified by column chromatography with silica gel

macroporous resin

Sephadex LH-20 and ODS-HPLC. The structures of the compound were identified by1H-NMR and13 C-NMR. Result: Seven compounds were isolated and identified as 1

7-dihydroxy-3

8-dimethoxyxanthone (1)

8-hydroxy-1

2

6-trime thoxyxanthone (2)

lancerin (3)

isovitexin (4)

iso-orientin (5)

morroniside (6)

6'-O-β-D-glucopyranosylmorroniside (7). Conclusion: Compounds 3

6

7 are isolated from the plants of T. cordatum for the first time. And these compounds have such activities of anti-bacteria

anti-inflammation

hepatoprotection

anticoagulation and platelet aggregation and protection of myocardial cell. This study provides a good reference for further studies for the pharmacodynamic material basis and the development and utilization of T. cordatum

and a certain basis for studying chemical genetic relationship of T. cordatum.