Objective: Clematidis Radix et Rhizoma from Ranunculaceae plant is a common traditional Chinese medicine in clinical practice. It has efficacies of eliminating wind and dampness

dredging collaterals to stop pain

and eliminating bone mineral

and can be used to treat many diseases

such as rheumatism and pharyngitis. Meanwhile

it was reported to have certain side effects. In recent years

some findings showed that its extract has an inhibitory effect on the proliferation of leukemia cells. This paper aims to study the chemical constituents from Clematidis Radix et Rhizoma and reveal its material base. Method: Clematidis Radix et Rhizoma were crushed into powder and extracted with ethanol. This residues were dispersed in H2O

and then extracted successively with petroleum ester

ethyl acetate and butanol. The compounds were isolated by column chromatography on D101 Macroporous resin

silica gel

Sephadex LH-20

MCI

and RP-18

and the structures were identified through spectroscopic methods

including 1H-NMR

13 C-NMR and MS. Result: Compounds 1-9 from this plant were identified as cimigenol (1)

cimigenol-3-O-β-D-xylpyranoside (2)

cimigoside (3)

friedelin (4)

(+)-3

3'-bisdemethylpinoresinol (5)

5-O-feruloyl-2-deoxy-D-ribono-γ-lactone (6)

ferulic acid (7)

syringic acid (8) and β-sitosterol (9). Conclusion: Compounds 1-6 were isolated from the rhizoma of Clematidis Radix et Rhizoma for the first time. Compounds 1-3 were cycloartane triterpenoid and obtained from Clematis genus for the first time. The isolated compounds further enrich chemical structure types from Clematidis Radix et Rhizoma

and offer a new basis to study plant taxonomy

quality control and pharmacological function.