Objective: To develop a certified reference material (CRM) of sakuranetin according to the Work Guideline for Reference Materials (3):Reference Material-General Principles and Statistical Method for Certification (GB/T 15000.3-2008) upon the approved by Standardization Administration of China (SAC). Method: Sakuranetin was extracted from dried barks of Populus tomentosa. The structure of sakuranetin was identified by optical rotation

elemental analysis

IR

UV

high-resolution MS

NMR

and X-ray diffraction analysis. Thin layer chromatography was carried out for the identification. Sakuranetin was divided into 400 bottles

with 5 mg per bottle. The homogeneity test

stability test

and quantitative analysis were carried out by high-performance liquid chromatography-ultra violet detector. Result: This CRM is DL-5

4'-dihydroxy-7-methoxyflavanone. The homogeneity of the 95% confidence interval was good. The storage period was 24 months at 0-8℃. The certified purity value was 99.97%

with a relative expanded uncertainty of 0.02%. The CRM of sakuranetin reached the technical requirements of CRM

and was accepted by SAC. Conclusion: The CRM of sakuranetin is successfully developed

and can be used for determining content

evaluating test methods

detecting relevant products and controlling quality.