Objective: To establish a highly sensitive and highly accurate method for simultaneous and fast multi-elements analysis

determinate the contents of 10 kinds of trace elements in Lepisorus ussuriensis which are essential to human by using this method

fill the blank of studying on the trace elements of this plant

and provide scientific basis for its quality control and further research. Method: Three batches of L. ussuriensis produced in Jilin Province were used as samples. The contents of trace elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) after the samples were diluted with wet digestion in HNO3-H2O2 system; then with In

Rh as the internal standard

the contents of elements were calculated with standard curve method. Result: For all of the analyzed elements

the standard curve correlation coefficients were all over 99.98%. RSDs were less than 3.8% and the recovery rates were between 94.8%-105.6%. The detection limit was 9×10-4 μg·g-1 for Co element

and were more than 1×10-3 μg·g-1 for other 9 elements. The linear range was 0.05-150 μg·g-1 for all the 10 trace elements

and the contents of elements Mn

Zn

Cr

Ni

Cu

V

Mo

Se

Co

Sn were 213.7

48.63

13.89

6.543

6.502

2.571

1.231

0.581 9

0.598 2

and 0.295 7 μg·g-1 respectively. Conclusion: The established rapid determination method has high sensitivity

wide linear range and good accuracy. L. ussuriensis has rich contents of Mn

Zn

Cr

Ni

Cu

V and other trace elements essential to human

and they may play direct or indirect effects on the efficacy of this plant when they participate in vivo process. This paper could provide some references for further research on elemental speciation

quality standard establishment and activity screening of L. ussuriensis.