Objective: To investigate the effect of Ershiwuwei Ercha pill in acute gouty arthritis rats induced by sodium urate crystals. Method: A total of 80 SD rats were randomly divided into blank group

model group

colchicine group (0.3 mg·kg-1)

hydrocortisone group (4 mg·kg-1) and twenty-five taste tea pill different dose group (2.4

1.2

0.6 g·kg-1). Except for blank group

all of the remaining groups were included into the acute gout arthritis model by injecting uric acid into the rat ankle joint. The joint swelling degree was measured after making model at 0

3

6

9

12

15.At the same time

white blood cell(WBC) and neutrophil(NEUT) were determined by analyzingblood cell from orbital venous plexus

and the levels of tumour necrosis factor-α(TNF-α)

interleukin-1β(IL-1β)

IL-8

cyclooxygenase-2(COX-2) in synovial tissues were determined by ELISA

and the expression of COX-2 protein in synovial tissues was detected by Western blot. Result: Compared with the blank group

the joint swelling degree of rats in the model group was significantly increased at each time point.The numbers of WBC and NEUT were significantly increased

the level of TNF-α

IL-1β

IL-8

COX-2 were significantly increased (P < 0.05

P < 0.01)

the expression of COX-2 protein were slightly increased

but with no statistical difference. Compared with the model

the joint swelling degree of rats in the high

middle and low-dose Ershiwuwei Ercha pill groups were significantly reduced

with a significant increase in inhibition ratio.The numbers of WBC and NEUT were significantly reduced. The level of TNF-α

IL-1β

IL-8

COX-2 were significantly decreased.The expression of COX-2 protein were significantly reduced(P < 0.05

P < 0.01). Conclusion: Ershiwuwei Ercha pill can reduce joint swelling degree

leukocyte

neutrophil cell number and inflammatory factors release to resist inflammatory reaction.