Objective: Based on constituents knock-out strategy

the preliminary recognition of main anti-dysmenorrhea effect components in Cyperi Rhizoma with Jianchangbang processing method was investigated. Method: Silica gel column chrppmatography and preparative HPLC were used

the main active ingredients of anti-dysmenorrha [α-cyperone(A)

cyperotundone(B) and sugeonol(C)] in the petroleum ether part of Cyperi Rhizoma were knocked out

according to the method knocked out the component of A

B

C

AB

AC

BC

ABC in sequence

thus got the target components of A+

B+

C+

AB+

AC+

BC+

ABC+and its corresponding negative samples of A-

B-

C-

AB-

AC-

BC-

ABC-.The target compositions and corresponding negative samples were detected by HPLC

through the test of uterine smooth muscle contraction from isolated mouse uterus

to explore influence of various target compositions and corresponding negative sample

petroleum ether on the isolated mouse uterine smooth muscle. Result: Combination of α-cyperone

cyperotundone and sugeonol on the goal of the composition of anti-dysmenorrhea effect was better than separate on the goal of composition;compared with petroleum ether part

anti-dysmenorrhea effect of the negative samples A-

B-

C-decreased obviously;the anti-dysmenorrhea effect of AB+was significantly better than the knocked-out negative sample AB-;the knocked-out negative sample ABC-still remained a certain of anti-dysmenorrhea effect;the summary of average inhibiting rate in each target components and corresponding negative samples were more than in petroleum ether part. Conclusion: Cyperotundone

α-cyperone and sugeonol are anti-dysmenorrhea effect components in Cyperi Rhizoma based on the whole level of traditional Chinese medicine

and there is a synergy relationship among them

but there are other components about anti-dysemenorrhea effect in Cyperi Rhizoma except these three components.There may be an antagonistic effect between target components and corresponding negative samples (mixture of other components).