Objective: To establish a HPLC-QQQ-MS method for simultaneous determination of six alkaloids in processed Aconiti Lateralis Radix Praeparaia from Fuzi Lizhong Wan. Method: Thermo Accucore column (2.1 mm×100 mm

2.6 μm) was adopted and eluted with mobile phase of 0.1% formic acid in water-0.1% formic acid in acetonitrile

with a flow rate at 0.3 mL·min-1. The column temperature was set at 30 ℃. The detection was accomplished in the positive ionization mode with scanning viaelectrospray ionization (ESI)

the scanning mode was multiple-reaction monitoring (MRM). According to the quantitative analysis

the ions were benzoylmesaconine m/z 105.0

benzoylaconitine m/z 105.0

benzoylhypaconitine m/z 542.3

mesaconitine m/z 572.3

aconitine m/z 586.3 and hypaconitine m/z 556.3. Result: Benzoylmesaconine

benzoylaconitine

benzoylhypaconitine

mesaconitine

aconitine and hypaconitine had a good linearity in the ranges of 0.184 80-1.848 ng (r=0.999 6)

0.035 36-0.353 6 ng (r=0.999 6)

0.129 60-1.296 0 ng (r=0.999 8)

0.011 65-0.116 5 ng (r=0.999 8)

0.002 95-0.029 5 ng (r=0.999 8)

0.036 96-0.369 6 ng (r=0.999 8)

respectively. The recoveries for the six analytes were between 91.93% and 106.70%

with RSD less than 6%. Conclusion: This method is simple

accurate

sensitive and repeatable

and can be used to determine content of alkaloids in processed Aconiti Lateralis Radix praeparaia from Fuzi Lizhong Wan.