Objective: To discuss the effect of thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma on hemorheology of dysmenorrhea rats caused by oxytocin and to investigate its in vitro transdermal absorption properties. Method: SD rats were randomly divided into 4 groups

such as the blank group

model group

thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma group and petroleum ether extract of Jianchangbang Cyperi Rhizoma group.The dysmenorrhea rats model were made by oxytocin and diethylstilbestrol

then were observed the times and incidence of wrings within 30 minutes.The blood viscosity and plasma viscosity of rats in all groups was tested by automatic blood rheometer.The percutaneous permeation of α-cyperone and cyperotundone in the thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma was studied by in vitro transdermal delivery experiment with Franz diffusion cells and their contents were determined by HPLC. Result: Thermal moxibustion emplastrum group and petroleum ether extract group could significantly reduce the times and incidence of wrings within 30 minutes

and the thermal moxibustion emplastrum group was better than the petroleum ether-extract group.Compared with the blank group

the model group significantly increased the whole blood viscosity and plasma viscosity

but the thermal moxibustion emplastrum group and the petroleum ether-extract group could significantly reduce them of model rats

also the thermal moxibustion emplastrum was better.The permeation rates of α-cyperone and cyperotundone from the thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma were 45.42

58.58 μg·cm-2·h-1

and their transdermal behavior conformed to Higuchi equation. Conclusion: Thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma can significantly improve the drug release properties of active ingredients with auxiliary heating materials

it can effectively relieve oxytocin-induced pain in rats and improve hemorheology of dysmenorrhea rats.