Objective: To compare the differences in ITS sequence of Salviae Miltiorrhizae Radix et Rhizoma from different origins

analyze the relationship between ITS sequence

effective composition content and origins by detecting its effective composition content

and lay a foundation for exploring the quality differences mechanism of Salviae Miltiorrhizae Radix et Rhizoma from different regions. Method: DNA was extracted from the Salviae Miltiorrhizae Radix et Rhizoma from different origins

and PCR-sequencing technology was adopted

then the sequence analysis software was used to analyze the gene sequence differences between different origins. UPLC method was used to measure the effective composition content in Salviae Miltiorrhizae Radix et Rhizoma from different origins. Result: It was found that there were some differences in ITS sequences among different origins

then the ITS sequences after being induced by the elicitor showed two types of obvious mutations (A and G); in addition

the effective composition content also showed obvious differences between different origins. Conclusion: It was concluded that the mutations at 152 bp of Salvia Miltiorrhizae Radix et Rhizoma induced by different elicitors could lead to more ‘G’-shaped transformation. The water-soluble constituents of Salvia Miltiorrhizae Radix et Rhizoma were increased after being processed by chitosan and salicylic acid respectively. No significant effect on the increase of the active ingredients was found after combined induction by chitosan and salicylic acid.