Objective: To determine and compare the contents of congmunoside TTP and congmunoside V in Aralia elata leaves from different habitats and gathering periods. Method: HPLC-ELSD was carried out on a Diamonsil C18 (2) column (4.6 mm×250 mm

5 μm) at a column temperature of 30 ℃. The mobile phase was acetonitrile and water (31: 69) at flow rate of 1.0 mL·min-1. The detector was ELSD

with the temperature of drift tube set at 110 ℃

and the flow rate of carrier gas (N2) was 3.2 mL·min-1. Result: The linear ranges of congmunoside TTP and congmunoside V were 0.51-2.55 μg (r=0.999 8) and 0.50-4.00 μg (r=0.999 2)

respectively. According to the results

the contents of congmunoside TTP and congmunoside V in A. elata leaves from different habitats and gathering periods were different. This may be related to distribution areas

growing seasons and physiological state of leaves. On the basis of the experimental results

the content of two kinds of ingredients was high mostly in July and August

and partially in September. Conclusion: The method is simple

rapid and accurate for determining simultaneously the contents of congmunoside TTP and congmunoside V in A. elata leaves

and can provide a basis for the quality control of A. elata leaves.