Objective: To establish a UPLC method for the simultaneous determination of aesculin

salidroside

aesculetin

syringin

fraxin

fraxetin and scopoletin in the Fraxini Cortex formula granule

in order to compare the difference of the seven chemical constituents in various Fraxini Cortex formula granule samples and provide an effective method to ensure the scientific quality of Fraxini Cortex formula granule. Method: The separation was performed on a Waters ACQUITY UPLC BEH C18 column (2.1 mm×50 mm

1.7 μm)

and eluted with mobile phase consisting of methanol-0.1% phosphoric acid solution in a gradient mode (0-1 min

5%-10%A; 1-10 min

10%-12%A) at a flow rate of 0.4 mL·min-1. The sample size was 2 μL. The detection wavelength was 220 nm

and the column temperature was 38 ℃. Result: Peak area and concentration for aesculin

salidroside

aesculetin

syringing

fraxin

fraxetin and scopoletin showed a good linearity within their ranges

the average recoveries of the chemical constituents were 100.0% (RSD 2.0%)

99.5% (RSD 2.1%)

99.9% (RSD 1.5%)

99.7% (RSD 2.2%)

99.4% (RSD 1.4%)

100.8% (RSD 1.8%)

99.4% (RSD 2.6%)

respectively. Conclusion: The established method is simple and accurate

and can be used to simultaneously determine seven chemical constituents in Fraxini Cortex formula granule

and the analysis time was significantly shortened. It's the first time to make a quantitative determination for many ingredients except for aesculin and the aesculetin in the Fraxini Cortex formula granule. There are certain differences in the contents of chemical compositions in the Fraxini Cortex formula granule of various batches and manufacturers.