Objective: To establish a HLC fingerprint of the compounds in Fuke Qianjin tablets and make a multi-component quantitative determination，in order to provide a reference for quality control. Method: Sunfire C18 column (4.6 mm × 250 mm，5 μm) was used，with a mobile phase of 0.1% phosphoric acid and acetonitrile gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was 326 nm from 0 to 12 min，316 nm from 12 to 18 min，225 nm from 18 to 24 min and 254 nm from 24 to 50 min. The column temperature was 30 ℃，and the sample size was 10 μL. Similarity evaluation and cluster analysis (CA) were performed to evaluate the fingerprint of the 11 batches of Fuke Qianjin tablets. Result: The common mode of the fingerprint included 14 common peaks，and 5 of them were identified by comparison with reference substances as chlorogenic acid，ferulic acid，andrographolide，berberine hydrochloride and dehydroandrographolide. The similar degrees of the 11 batches of samples were more than 0.90. Conclusion: The established method has a high sensitivity and specificity，and can be used for quality control of Fukeqianjin tablets.