黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较

邹淑君; 许树军; 李靖; 吕邵娃; 肖洪彬

doi:10.13422/j.cnki.syfjx.2017120033

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黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较

资源与鉴定 | 更新时间：2024-01-04

- 黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较
- Content of Two Saponins in Leaves of from Different Altitudes and Different Gathering Periods of Fenghuang Mountain
- 中国实验方剂学杂志 2017年23卷第12期页码：33-37
- 作者机构：
- 作者简介：
- 基金信息：
  
  中国博士后科学基金项目（2014M551289）;国家“重大新药创制”科技重大专项（2011ZX09102-001-18）
- DOI：10.13422/j.cnki.syfjx.2017120033
  中图分类号： R284.1
- 纸质出版日期：2017
- 稿件说明：
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邹淑君, 许树军, 李靖, 等. 黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较[J]. 中国实验方剂学杂志, 2017,23(12):33-37.

ZOU Shu-jun, XU Shu-jun, LI Jing, et al. Content of Two Saponins in Leaves of from Different Altitudes and Different Gathering Periods of Fenghuang Mountain[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 33-37.
邹淑君, 许树军, 李靖, 等. 黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较[J]. 中国实验方剂学杂志, 2017,23(12):33-37. DOI： 10.13422/j.cnki.syfjx.2017120033.

ZOU Shu-jun, XU Shu-jun, LI Jing, et al. Content of Two Saponins in Leaves of from Different Altitudes and Different Gathering Periods of Fenghuang Mountain[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 33-37. DOI： 10.13422/j.cnki.syfjx.2017120033.

摘要

目的：比较黑龙江凤凰山地带不同海拔及不同采收期辽东楤木叶中三萜皂苷3-O-[β-D-吡喃葡萄糖基（1→2）][β-D-吡喃葡萄糖基-（1→3）]-β-D-吡喃葡萄糖基-刺囊酸-28-O-β-D-吡喃葡萄糖酯（简写为ECA）和3-O-β-D-吡喃葡萄糖基-（1→3）-β-D-吡喃葡萄糖基-（1→3）-β-D-吡喃葡萄糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖酯（以下简称为OLA）的含量，了解这一区域采收辽东楤木叶的最佳时间和海拔高度。方法：用反相高效液相色谱-蒸发光散射检测（HPLC-ELSD）法测试，色谱柱为Diamonsil C18（4.6 mm×250 mm，5 μm），柱温30℃；流动相为乙腈、水二元梯度系统；进样量10 μL，流速1.0 mL·min-1；载气流速3.2 L·min-1；漂移管温度110℃。结果：两种三萜皂苷在各自测定的范围内均呈良好线性关系（r均为0.999 8），平均加样回收率分别为97.71%，98.06%。各批次供试品中OLA的含量都远高于ECA。从6月初至9月初，两种皂苷含量几乎都呈递增趋势，6月至8月增幅很大，差别显著，8月至9月增幅较小，甚至略减；不同海拔高度条件下同时期的叶中两种皂苷含量没有显著性差异。结论：所用测试方法简单、快捷、重复性好，可为凤凰山地带辽东楤木叶的质量控制提供依据。

Abstract

Objective: To compare the contents of 3-O-[β-D-glucopyranosyl-(1→2)] [β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl echinocystic acid-28-O-β-D-glucopyranosyl ester (ECA for short) and 3-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl oleanolic acid-28-O-β-D-glucopyranosyl ester (OLA for short) in leaves of Aralia elata collected from Fenghuang mountain of Heilongjiang Province in different altitudes and different gathering periods. Method: HPLC-ELSD was used to determine the content. The column was Diamonsil C18 (4.6 mm×250 mm

5 μm). The column temperature was 30℃. The mobile phase was the mixture liquid of acetonitrile and water for gradient elution. The sample size was 10 μL

and the flow rate was 1.0 mL·min-1. The detector was HPLC-ELSD

the temperature of drift tube was 110℃

and the flow rate of carrier gas was 3.2 L·min-1. Result: The two triterpenoid saponins showed a good linearity (r=0.999 8) within their detection range

and the average recovery rate were 97.71% and 98.06%

respectively. The results showed that the content of OLA was higher than ECA in various batches of samples. From June to September

the contents of the two saponins showed an increasing trend. The contents increased significantly from June to August

but slowly from August to September. In addition

there were no significant difference in content of the two saponins between high altitude and low altitude. Conclusion: The method is simple

rapid and reproducible

and can provide a basis for the quality control of leaves of A. elata from Fenghuang Mountain.

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