Objective: To analyze and identify flavonoids in Rosae Laevigatae Fructus Midhx by using UPLC-Q-TOF-MS. Method: ACQUITY UPLC BEH C18 column(2.1 mm×50 mm

1.7 μm) was adopted and eluted with 0.1% formic acid and 0.1% acetonitrile as the mobile phase

with the flow rate at 0.3 mL·min-1

the column temperature was 35℃

and the injection volume was 5 μL. The collected data was monitored under positive and negative electrospray ion modes. Meanwhile

flavonoids were identified with quasi-molecular ions and secondary fragment ions. Result: Ten flavonoids were identified based on their elemental compositions

MS/MS data and published literatures through rapid separation of ethanol extraction solution with UPLC

which were icariin

kaempferitrin

6-methoxykampferol-3-O-galactoside

apigenin

hesperidin

hyperoside

rutinum

liquiritigenin

potengriffioside A and quercetin. Conclusion: UPLC-Q-TOF-MS is a rapid and effective technology to analyze flavonoids in Rosae Laevigatae Fructus

and provides an accurate and effective method for the identification of components

the basis for quality control and a reference for defining the material basis of its efficacy.