Objective: To investigate the intervention effect of Bushen Huatan Yizhi decoction(BSHT) in APPV717I transgenic mice and explore its possible mechanism. Method: The 120 three months old APPV717I transgenic mice were randomly divided into two batches

n=60 in each batch. The mice in each batch were randomly divided into model group (APP)

Donepezil group (0.000 92 g·kg-1·d-1)

BSHT low dose group(5.2 g·kg-1·d-1)

middle dose group(10.4 g·kg-1·d-1)

and high dose group(20.8 g·kg-1·d-1)

n=12 in each group (6 males and 6 females). Another 24 C57BL/6J mice of the same age were set as a normal group

and the mice in model group and normal group received intragastric administration of equivalent volume of distilled water

1 time/day. The first batch of mice received drug administration for 8 months

and the second batch for 4 months. Behavioral ability was measured in Morris water maze finally

and the expression levels of Tau protein hosphorylation (p-Ser 199/202

Tau-1) in the hippocampus was determined by using enzyme linked immunosorbent assay(ELISA) and Western blot. Result: Behavioral experiment results showed that

as compared with the normal group

the latency was prolonged in model group mice(P<0.05

P<0.01). After months treatment

the latency was shortened in various treatment groups as compared with model group(P<0.05). ELISA and Western blot assay showed that as compared with the normal group

the phosphorylation degree of Ser199/202 phosphorylation sites in hippocampal neurons was increased significantly in model group(P<0.01); and the phosphorylation degree of Ser199/202 phosphorylation sites in various treatment groups was significantly lower than that in model group(P<0.05

P<0.01). Conclusion: Bushen Huatan Yizhi decoction can improve the phosphorylation degree of Ser199/202 phosphorylation sites

prevent neurofibrillary tangle(NFT) formation

and thus improve the early learning and memory abilities in APPV717I transgenic mice.