赤雹根总皂苷对II型胶原诱导性关节炎大鼠IL-15,IL-34表达的影响

田沂凡; 佟继铭; 马帅; 刘永平

doi:10.13422/j.cnki.syfjx.2017120126

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赤雹根总皂苷对II型胶原诱导性关节炎大鼠IL-15,IL-34表达的影响

药理 | 更新时间：2024-01-04

- 赤雹根总皂苷对II型胶原诱导性关节炎大鼠IL-15,IL-34表达的影响
- Effect of Total Saponins of Root on Expression of IL-15, IL-34 in Rats with Arthritis Induced by Type II Collage
- 中国实验方剂学杂志 2017年23卷第12期页码：126-131
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省科技厅支撑项目（14272502D）;河北省教育厅重大项目（ZD2016013）;河北省教育厅自然科学项目（277）
- DOI：10.13422/j.cnki.syfjx.2017120126
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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田沂凡, 佟继铭, 马帅, 等. 赤雹根总皂苷对II型胶原诱导性关节炎大鼠IL-15,IL-34表达的影响[J]. 中国实验方剂学杂志, 2017,23(12):126-131.

TIAN Yi-fan, TONG Ji-ming, MA Shuai, et al. Effect of Total Saponins of Root on Expression of IL-15, IL-34 in Rats with Arthritis Induced by Type II Collage[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 126-131.
田沂凡, 佟继铭, 马帅, 等. 赤雹根总皂苷对II型胶原诱导性关节炎大鼠IL-15,IL-34表达的影响[J]. 中国实验方剂学杂志, 2017,23(12):126-131. DOI： 10.13422/j.cnki.syfjx.2017120126.

TIAN Yi-fan, TONG Ji-ming, MA Shuai, et al. Effect of Total Saponins of Root on Expression of IL-15, IL-34 in Rats with Arthritis Induced by Type II Collage[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(12): 126-131. DOI： 10.13422/j.cnki.syfjx.2017120126.

摘要

目的：观察赤雹根总皂苷（total saponins of Thladiantha dubia root，TSTR）治疗牛II型胶原诱导性关节炎（CIA）大鼠，血液及膝关节滑膜中破骨细胞（OC）正反馈因子白细胞介素-15（IL-15），IL-34的水平变化，探讨TSTR治疗大鼠CIA的作用机制。方法：雄性Wistar大鼠80只，随机取10只作为正常组，其余大鼠均于尾根部及背部皮内多点注射牛II型胶原与完全弗氏佐剂混匀的乳剂。7 d后同法操作，以首次注射后14 d，大鼠关节炎指数（AI值）≥4分为造模成功，将模型大鼠随机分为模型组，TSTR低、中、高剂量组（20，40，80 mg·kg-1）和雷公藤多苷（TG）阳性药组（12 mg·kg-1），各药物组均灌胃给予相应药物35 d，每天1次。观察大鼠足爪肿胀度，评价AI值；采用苏木精-伊红染色法（HE）观察滑膜病理变化；酶联免疫吸附测定（ELISA）检测大鼠血清中IL-15，IL-34含量；逆转录-聚合酶链式反应（RT-PCR）检测大鼠滑膜中IL-15，IL-34 mRNA表达水平；蛋白质印迹法（Western blot）检测滑膜中IL-15，IL-34蛋白表达水平。结果：与正常组比较，模型组大鼠AI值显著增高（P<0.01），滑膜细胞中炎性细胞的数量增多，在血清和滑膜组织中，IL-15，IL-34的含量、基因和蛋白表达均显著增高（P<0.01）；与模型组比较，TSTR各剂量组和TG组AI值显著降低（P<0.01）；且TSTR低剂量组和TG组滑膜中炎性细胞数量及血管数量明显减少；TSTR低剂量组和TG组大鼠血清中IL-15，IL-34表达和滑膜中IL-15，IL-34蛋白表达，IL-15，IL-34 mRNA的表达均显著降低（P<0.05，P<0.01）。结论：TSTR对CIA大鼠的治疗作用，可能与抑制血液和滑膜组织中IL-15，IL-34的表达有关。

Abstract

Objective: To observe the effect of total saponins of Thladiantha dubia root(TSTR) on changes of interleukin-15(IL-15) and interleukin-34(IL-34) in the blood and knee synovial membrane in the treatment of type II collagen-induced arthritis (CIA) rats

and explore the mechanism of TSTR in treating CIA rats. Method: The 80 male Wistar rats were selected

and ten of them were randomly taken as normal control group. The remaining rats were injected with the mixed emulsion of bovine type II collagen and complete Freund's adjuvant in multiple points on back and tail root. After 7 days

it was carried out with the same operation.Fourteen days after the first injection

those with arthritis index (AI value)≧4 points were identified as successful modeling. The successfully modeled rats were randomly divided into model group

TSTR low

medium and high dose groups(20

80 mg·kg-1) and tripterygium glycoside(TG

12 mg·kg-1) positive drug group. Rats in each group received corresponding drugs for 35 days by intragastric administration

1 time/day. Then the paw swelling in rats was observed and AI values were evaluated; the morphological changes of synovium were observed with htoxylin eosin(HE) staining; the levels of IL-15 and IL-34 in serum were detected by enzyme-linked immunosorbent assay(ELISA); Western blot and reverse transcriptase PCR(RT-PCR) were used respectively to measure the protein and mRNA expression levels of IL-15

IL-34 in synovium. Result: As compared with the normal group

AI value was increased in the model group (P<0.01); the number of inflammatory cells in synovial cells was increased

and the levels of IL-15 and IL-34 in serum and synovial tissues were significantly increased (P<0.01). As compared with the model group

AI value was significantly decreased in each dose TSTR groups and TG positive group(P<0.01)

and the inflammatory cells number and vascular quantity in the synovial membrane were significantly decreased in TSTR low dose group and the positive drug group (P<0.05

P<0.01); the expression levels of IL-15 and IL-34 in the serum and the expression levels of IL-15 and IL-34 in the synovial membrane were significantly decreased in the low-dose TSTR group and the TG positive group (P<0.05

P<0.01). Conclusion: The therapeutic effect of TSTR on CIA rats may be related to the inhibition of IL-15 and IL-34 expression in blood and synovium.

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