Objective: To investigate absorption characteristics of ferulic acid and ligustilide in Chuanxiong Rhizoma at different intestinal segment

and to explore the absorption changes of monomer and herbal extract

then to investigate the influence of P-glycoprotein(P-gp) and multidrug resistance-associated protein (MRP) on absorption of these two ingredients. Method: In situ single pass intestinal perfusion model was used and the change of concentration of ferulic acid and ligustilide were determined by UHPLC at various intestinal segments

different concentration of herbal extract

containing protein inhibitor

their absorption rate parameters (Ka) and effective permeability coefficients (Peff) were calculated. Result: Ferulic acid and ligustilide were absorbed in all intestinal segments

the absorption of ligustilide was better than ferulic acid.The Ka of ferulic acid in the duodenum was 1.23×10-3 s-1 and there was no significant difference in the jejunum and ileum.There was no significant difference about the absorption of ferulic acid in concentration of herbal extract with 12.5

20.0

40.0 g·L-1

but the absorption at 100 g·L-1 was significantly decreased compared with the former three mass concentration.Absorption of ferulic acid in herbal extract was significantly higher than monomer;compared with the extract group

its absorption in probenecid group was significantly increased.The Ka of ligustilide in the duodenum was 3.58×10-3 s-1

the order of absorption was ileum>duodenum≈jejunum>colon.The absorption of ligustilide decreased with increasing of mass concentration of herbal extract and significantly increased in groups containing verapamil and probenecid by comparing with the extract group

and the absorption of probenecid group was better than verapamil group. Conclusion: The absorption way of ferulic acid may be facilitated diffusion

existing carrier to participate in

ferulic acid is the substrate for MRP.Ligustilide mainly relies on the carrier of active transport

it may be substrates of P-gp and MRP

and it is greatly influenced by MRP.