Objective: To analyze difference of chemical ingredients between Aconiti Lateralis Radix Praeparata in Jiangyou and Anxian by UPLC-Q-TOF/MS. Method: UPLC-Q-TOF/MS was carried out to acquire chemical constituents informations from Aconiti Lateralis Radix Praeparata samples in Jiangyou and Anxian.High precise mass data were filtered and standardized processed by MassHunter B. 05.00 software and MetaboAnalyst 3.0 software

then these data were processed by multivariate statistical analysis techniques by SIMCA-P 13.0 software to discover and identify the constituents with significant difference. Result: Sixteen chemical markers with significant difference were screened by partial least squares discriminant analysis (PLS-DA).During which

the contents of mesaconitine

aconitine

chasmanine

talatisamine

benzoylmesaconitine

benzoylhypaconitine

dehydrated benzoylmesaconitine and mesaconine were higher in Aconiti Lateralis Radix Praeparata from Anxian

while the contents of neoline

fuziline

hypaconitine

talatidine

carmichaeline

fuzitine and songorine were much higher in Aconiti Lateralis Radix Praeparata from Jiangyou.The formation of this difference is related to the difference in cultivation conditions between these two places. Conclusion: Comprehensive analysis for the toxicity and activity of chemical markers in Aconiti Lateralis Radix Praeparata from these two places

the toxicity

analgesic and anti-inflammatory activity of samples in Anxian are much stronger

while the cardiac activity of samples in Jiangyou may be stronger.