Objective: To find out the impacting factors on uncertainty by establishing a HPLC method for a quantitative analysis of agarotetrol

in order to provide the evidence for quality control of traditional Chinese medicinal materials of Aquilariae Lignum Resinatum. Method: Agarotetrol in Aquilariae Lignum Resinatum was determined by HPLC

and the uncertainty was evaluated according to JJF1059.01-2012.The uncertainty components were quantified through a statistical analysis

and extended uncertainty and confidence level were finally determined. Result: The uncertainty evaluation results for the content determination of agarotetrol from five samples was (2.80±0.45)

(5.13±0.82)

(13.86±2.21)

(74.86±11.87)

(125.71±19.94)g·L-1. Sample homogeneity

weighing

compound concentration and HPLC property were the major factors to measure the uncertainty. The error of sample trace analysis could be reduced by quartering samples

drying weighing medium and correctly or regularly using glassware and precision instruments. Conclusion: The method can be used for uncertainty analysis of content of agarotetrol in Aquilariae Lignum Resinatum

which makes the standard trace amount more rational and significant to establish uncertainty analysis methods and quality standard for Chinese medicinal materials of Aquilariae Lignum Resinatum.