HPLC测定秦艽配伍药对前后特征性成分的变化

刘飞; 罗奎元; 马腾茂; 杨秀娟; 高慧琴

doi:10.13422/j.cnki.syfjx.2017130092

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HPLC测定秦艽配伍药对前后特征性成分的变化

化学与分析 | 更新时间：2024-01-04

- HPLC测定秦艽配伍药对前后特征性成分的变化
- Determination of Changes of Characteristic Components Before and After Different Gentiana Combinations by HPLC Method
- 中国实验方剂学杂志 2017年23卷第13期页码：92-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金地区基金项目（81360648）
- DOI：10.13422/j.cnki.syfjx.2017130092
  中图分类号： O657.72;R284
- 纸质出版日期：2017
- 稿件说明：
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刘飞, 罗奎元, 马腾茂, 等. HPLC测定秦艽配伍药对前后特征性成分的变化[J]. 中国实验方剂学杂志, 2017,23(13):92-97.

LIU Fei, LUO Kui-yuan, MA Teng-mao, et al. Determination of Changes of Characteristic Components Before and After Different Gentiana Combinations by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(13): 92-97.
刘飞, 罗奎元, 马腾茂, 等. HPLC测定秦艽配伍药对前后特征性成分的变化[J]. 中国实验方剂学杂志, 2017,23(13):92-97. DOI： 10.13422/j.cnki.syfjx.2017130092.

LIU Fei, LUO Kui-yuan, MA Teng-mao, et al. Determination of Changes of Characteristic Components Before and After Different Gentiana Combinations by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(13): 92-97. DOI： 10.13422/j.cnki.syfjx.2017130092.

摘要

目的：探讨药对秦艽-威灵仙、秦艽-桑寄生、秦艽-防己配伍前后特征性成分变化的规律。建立HPLC测定方法。方法：Agilent A3000250×046 PurFuit 5 C18色谱柱（4.6 mm×250 mm，5 μm），流动相0.04% H3PO4水溶液（A）-乙腈（B），梯度洗脱（0~15 min，5%~15% B；15~25 min，15%~25% B；25~35 min，25%~35% B；35~40 min，35%~70% B；40~55 min，70%~95% B），流速0.8 mL·min-1，检测波长220 nm，柱温25 ℃。结果：药对秦艽-威灵仙中分离出16个特征峰（w1~w16），秦艽-桑寄生中分离出18个特征峰（s1~s18），秦艽-防己中分离出21个特征峰（f1~f21），其中峰号w1，w3，w4，w5，w6，w7，w8，w10，w11，w13，w16，s3，s4，s5，s7，s9，s10，s11，s12，s13，s14，f1，f4，f6，f8，f12，f14，f15，f16，f21峰面积升高，w2，w9，s1，s2，s6，s8，f2，f3，f5，f10，f11，f13，f17，f19峰面积降低，w14，w15，s16，s17，s18，f9，f20峰面积不变，其中w8，s7，f9为獐芽菜苦苷，w10，s8，f11为龙胆苦苷，s13，f16，f17分别为槲皮苷、防己诺林碱、粉防己碱。w12，s15，f7，f18为药对中新产生的成分。结论：秦艽配伍用药后所含的特征性成分含量发生了变化且有新成分的生成，本研究所建立的HPLC分析秦艽不同配伍药对前后特征性成分变化的方法简便、准确、重复性较好。

Abstract

Objective: To establish an HPLC method for determination of changes of characteristic components before and after the compatibility of Gentianae Macrophyllae Radix-Clematidis Radix et Rhizoma

Gentianae Macrophyllae Radix-Taxilli Herba

and Gentianae Macrophyllae Radix-Stephaniae Teerandrae Radix. Method: The chromatographic column was Agilent A3000250×046 Pursuit 5 C18 (4.6 mm×250 mm

5 μm)

with 0.04% phosphoricacid solution (A)-acetonitrile (B) as the mobile phase for gradient elution (0-15 min

5% -15% B;15-25 min

15% -25% B;25-35 min

25% -35% B;35-40 min

35% -70% B;40-55 min

70% -95% B) at the flow rate of 0.8 mL·min-1. The detection wavelength was set at 220 nm

and the column temperature was set at 25 ℃. Result: The 16 characteristic peaks (w1-w16) were isolated from Gentianae Macrophyllae Radix-Clematidis Radix et Rhizoma herbal pair;18 characteristic peaks (s1-s18) were isolated from Gentianae Macrophyllae Radix-Taxilli Herba herbal pair;and 21 characteristic peaks (f1-f21) were isolated from Gentianae Macrophyllae Radix-Stephaniae Teerandrae Radix herbal pair. Among them

the peak areas of w1

w10

w11

w13

w16

s10

s11

s12

s13

s14

f12

f14

f15

f16 and f21 were increased;peak areas of w2

f10

f11

f13

f17 and f19 were decreased;and the peak areas of w14

w15

s16

s17

s18

f9 and f20 were not changed. w8

s7 and f9 were swertiamarin;w10

s8 and f11 were gentiamarin;s13

f16 and f17 were quercitin

fangchinoline and tetrandrine. w12

s15

f7 and f18 were the new components produced in the herbal pairs. Conclusion: After Gentianae Macrophyllae Radix compatibility

the contents of characteristic ingredient were changed and new components were generated. The established HPLC method was simple

accurate and repeatable to analyze the changes of characteristic components before and after different Gentianae Macrophyllae Radix drug combinations.

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