Objective: To explore the effects of serum containing Juglans mandshurica (SCJM) pericarp on proliferation and apoptosis of gastric carcinoma SGC-7901 cells. Method: Wistar rats were randomly divided into blank control group

low (25 g·kg-1·d-1)

medium (50 g·kg-1·d-1) and high (100 g·kg-1·d-1) dose J.mandshurica treatment groups and cisplatin group (5 g·kg-1·d-1). The rats in treatment groups received lavage administration for 7 d

and the rats in blank control group received the equivalent volume of normal saline. 2 h after the last treatment

SCJM was prepared by carotid blood serum; cell proliferation

apoptosis rates

apoptosis index

levels of apoptosis related gene and protein expression were evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) colorimetry

flow cytometry

Hoechst staining

Real-time PCR and Western blot respectively. Result: SCJM could inhibit the proliferation of SGC-7901 cells (P<0.05) as compared with blank group

and 48 h after treatment by SCJM

early-and late-apoptosis rates

apoptosis index and mRNA levels of Bax and Bak were increased

but mRNA level of B-cell lymphoma-2 (Bcl-2) was decreased (P<0.05); the protein levels of β-catenin

Cyclin D1 and C-myc in SCJM-treatment groups were lower than those of control group (P<0.05). Conclusion: SCJM had inhibitory effect on proliferation of gastric carcinoma SGC-7901 cells

and the effect may be related to the induction of apoptosis and inhibition of Wnt/β-catenin pathway activation.