Objective: To explore the anti-gastric tumor effect and mechanism of Ganoderma polysaccharides based on in vitro and in vivo experiments. Method: In the in vitro experiment

MKN45 and AGS cells were used and randomly divided into blank control

cis-platinum

high

middle and low-dose Ganoderma polysaccharides (20

10

5 g·L-1) groups. Cell counting kit-8 (CCK-8) and flow cytometry methods were used to detect cell proliferation

and flow cytometry was used to detect apoptosis and cycle change

after incubation for 24 and 48 h. In the in vitro experiment

60 BALB/c naked mice were randomly divided into blank control

model

adriamycin

high

middle and low-dose Ganoderma polysaccharides (200

100

50 mg·kg-1) groups. The gastric tumor model was established by transplanting Walker-256 cell. One week later

each group was given the corresponding drugs. And 4 weeks later

the tumor size was measured

and Real-time PCR method was used to observe the changes in Bcl-2 assaciated X protein (Bax) and B-cell lymphoma-2 (Bcl-2) gene expressions. Result: High

middle and low-dose Ganoderma polysaccharides groups showed inhibition in MKN45 and AGS proliferation in 24

48 h (P<0.05). High and middle-dose Ganoderma polysaccharides could promote MKN45 and AGS cell apoptosis in 24 h(P<0.05)

and inhibit AGS cell cycle in G1 stage (P<0.05). In vivo experiment showed that high and middle-dose Ganoderma polysaccharides could inhibit the growth of gastric tumor. Meanwhile

the increase on Bax gene expression

and inhibition on Bcl-2 gene expression were observed. Conclusion: Ganoderma polysaccharides could inhibit the growth of gastric tumor in vitro and in vivo. And the mechanism is correlated with improvement on Bax gene expression

inhibition on Bcl-2 gene expression and promotion in tumor cell apoptosis.