Objective: To identify the chemical constituents of Zhizi Jinhua Wan(ZZJHW) by ultra-high-pressure liquid chromatography coupled with electrospray time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) for quality control of commercial preparations. Method: The assay was performed on a ACQUITY BEH C18 column(2.1 mm×100 mm

1.7 μm)using a gradient elution program with mixtures of acetonitrile and low concentration of formic acid aqueous solution as mobile phases at a flow rate of 0.4 mL·min-1. UPLC-Q-TOF-MS/MS was developed for rapid and high-throughput screening of the preliminary chemical profile of ZZJHW in both positive and negative ion modes

the main peaks were confirmed basically according to MS2 fragment ions combined with the relevant literature. Result: Based on the retention time

fragmentation patterns and literature reports

a total of 65 compounds were identified or tentatively characterized in ZZJHW

including 9 iridoids

8 organic acids

6 anthraquinones

20 flavonoids

4 steroidal saponins

et al. Conclusion: This work provides a good example for simultaneous analysis of different types of chemical constituents which may be helpful for the quality control and further phytochemical studies of ZZJHW.