Objective: To establish the ultra performance liquid chromatography (UPLC) fingerprint of Evodiae Fructus

and provide a comprehensive evaluation for their quality from different species and regions. Method: The UPLC method was performed on ACQUITY UPLC BEH C18 (2.1 mm×100 mm

1.7 μm)

with acetonitrile-0.1% formic acid aqueous solution as mobile phase for gradient elution at a flow rate of 0.4 mL·min-1. The detection wavelength was set at 300 and 215 nm

the column temperature was set at 35 ℃. Similarity evaluation

hierarchical clustering analysis and partial least squares-discriminate analysis were applied to evaluate the fingerprints of 37 batches of Evodiae Fructus. Result: The UPLC fingerprint and common mode were established with 34 common peaks

and 17 of them were identified based on the reference substances. The similarities of 37 samples were 0.703-0.973.And fingerprint profiles of various samples from different regions were basically similar

but the relative contents of components varied greatly. Based on the results of hierarchical clustering analysis and partial least squares-discriminate analysis

the relative contents of components were almost unrelated to the species and regions

but related to 1 000 grain-weight of the fruits to a certain extent. Rutaecarpine

evodiamine

neochlorogenic acid

chlorogenic acid

evocarpine and limonin turned out to be the top 6 differential compounds. Conclusion: This simple and rapid method could be effectively used for the comprehensive quality evaluation of Evodiae Fructus. It is the most important to confirm that near mature collection time is the key to guarantee the quality of crude drug of Evodiae Fructus.