Objective: To analyze the chemical differences of Smilacis Glabrae Rhizome from Vietnam and China

identify discrimination markers

and establish the method of identifying the origins of samples. Method: Samples were collected from Vietnam (7 batches) and China (28 batches). The chromatography fingerprints of the above samples were established

and the differences between the two kinds of samples were compared according to the similarity evaluation results. At the same time

the main chromatographic peaks were identified by HPLC-MS

and 21 peaks were identified. Cluster analysis was used to identify the origin of the samples. Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to determine the discrimination markers between samples collected from Vietnam and China

and the points with VIP > 1 at the ends of S-plot were selected as potential markers. Result: Differences in main chemical compounds were found between the samples from Vietnam and China. Thirty-five samples were divided into Vietnam group and China group. Astilbin

isoastilbin

neoastilbin and engeletin were determined to be the discrimination markers

and their contents in samples of Vietnam were obviously higher than those in samples of China. Conclusion: Fingerprint coupled with OPLS-DA can be used to analyze the chemical similarity and differences between samples from Vietnam and China. The results provided data not only for the quality evaluation of samples from Vietnam

but also for the answer on whether samples from Vietnam can be used as medicine.