Objective: To study the chemical constituents of Hoyalyi Levl

isolate and identify its components. Method: Hoyalyi Levl was extracted with 90% ethanol reflux

and Hoyalyi Levl concrete was obtained after low pressure concentration. The above concrete was dissolved with water by ultrasonic method

and then extracted in turn with petroleum ether

dichloromethane and n-butyl alcohol. The n-butanol extract was used for silica gel chromatography column

with petroleum ether-acetone- methanol (80:20:0-0:20:8 0) as the mobile phase for gradient elution

and then was purified with silica gel column and Sephadex LH-20 after crystallization

recrystallization and centrifugal separation. The dichloromethane extract was eluted with petroleum ether-acetone-methanol (50:50:0) gradient elution system

and then their same components were merged; the constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatographic techniques. ESI-MS

1H-NMR

and 13C-NMR were used to identify the structure of the isolated constituents. Result: Seven compounds were isolated from n-butyl alcohol fraction and 8 compounds were isolated from dichloromethane fraction

and their chemical structures were identified as p-hydroxyben-zaldehyde (1)

syringic acid (2)

benzoic acid (3)

tramcinnamic acid (4)

paeonol (5)

quercetin (6)

esculetin(7)

femlie acid (8)

p-hydroxybenzal acid (9)

2

3-dihydroxy-benzoic acid (10)

3

4-dihydroxybenzoic acid (11); oleanolic acid(12); hyperoside (13)

gallic acid(14) and β-sitosterol (15). Conclusion: All the compounds are isolated and identified from the Hoyalyi Levl for the first time.