Objective: To explore the biological basis of constipated mouse cured by optimal prescription of Da Chengqitang with differential proteomics. Method: Mice were randomly divided into normal group

model group and optimal prescription of Da Chengqitang group.Mice in the model group and optimal prescription of Da Chengqitang group were delivered compound diphenoxylate suspension with 50 mg·kg-1 to develop constipation model.While the normal mice were delivered the same volume of normal saline.Then

mice in optimal prescription of Da Chengqitang group were delivered with decoction at the dose of 31 g·kg-1

other mice were delivered the same volume of normal saline.When finishing the experiment

proteins of large intestine were extracted

digested and detected by NanoLC-LTQ-Orbitrap system.The data was imported into Protein Discovery software to identify the proteins and all the raw data was analyzed by Sieve v2.1 software. Result: Compared with the model group

77 significant difference proteins were found in optimal prescription of Da Chengqitang group

which the variation trend was consistent with the normal group.Literature retrieval and analysis showed that the biological functions of differential proteins focused on sugar and lipid metabolism

tricarboxylic acid cycle

biological oxidation and energy metabolism to provide energy for large intestine peristalsis and fortify contraction function of the intestinal smooth muscle. Conclusion: The above target proteins and their regulation pathways may be the biological basis of constipated mice cured by optimal prescription of Da Chengqitang.