基于Pink-1/Parkin信号通路补青颗粒含药血清对高糖诱导人晶状体上皮细胞线粒体自噬的影响

李文珊; 牛阳; 南一; 马会明; 阙平; 马琼; 范博研; 潘雪军

doi:10.13422/j.cnki.syfjx.2017140128

您当前的位置：

首页 >

文章列表页 >

基于Pink-1/Parkin信号通路补青颗粒含药血清对高糖诱导人晶状体上皮细胞线粒体自噬的影响

药理 | 更新时间：2024-01-04

- 基于Pink-1/Parkin信号通路补青颗粒含药血清对高糖诱导人晶状体上皮细胞线粒体自噬的影响
- Effect of Buqing Granules Medicated Sera on Mitochondrial Autophagy in High Glucose-induced Human Lens Epithelial Cells Based on Pink-1/Parkin Signaling Pathway
- 中国实验方剂学杂志 2017年23卷第14期页码：128-133
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81560749）
- DOI：10.13422/j.cnki.syfjx.2017140128
  中图分类号： R285
- 纸质出版日期：2017
- 稿件说明：
移动端阅览
李文珊, 牛阳, 南一, 等. 基于Pink-1/Parkin信号通路补青颗粒含药血清对高糖诱导人晶状体上皮细胞线粒体自噬的影响[J]. 中国实验方剂学杂志, 2017,23(14):128-133.

LI Wen-shan, NIU Yang, NAN Yi, et al. Effect of Buqing Granules Medicated Sera on Mitochondrial Autophagy in High Glucose-induced Human Lens Epithelial Cells Based on Pink-1/Parkin Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 128-133.
李文珊, 牛阳, 南一, 等. 基于Pink-1/Parkin信号通路补青颗粒含药血清对高糖诱导人晶状体上皮细胞线粒体自噬的影响[J]. 中国实验方剂学杂志, 2017,23(14):128-133. DOI： 10.13422/j.cnki.syfjx.2017140128.

LI Wen-shan, NIU Yang, NAN Yi, et al. Effect of Buqing Granules Medicated Sera on Mitochondrial Autophagy in High Glucose-induced Human Lens Epithelial Cells Based on Pink-1/Parkin Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(14): 128-133. DOI： 10.13422/j.cnki.syfjx.2017140128.

摘要

目的：探讨补青颗粒含药血清对高糖诱导的人晶状体上皮（HLE）细胞线粒体自噬的影响。方法：体外培养HLE细胞，空白组将HLE细胞在正常培养液中培养48 h，模型组在空白组培养液中添加30 mmol·L-1葡萄糖，实验组在模型组基础上加入不同含药血清刺激培养48 h。采用CCK-8法检测不同含药血清对高糖诱导的HLE细胞增殖活力的影响；利用免疫荧光技术及蛋白免疫印迹（Western blot）法检测Pink-1，Parkin，p62蛋白表达。结果：CCK-8法结果显示模型组较空白组HLE细胞增殖活力增强（P<0.01）；与模型组比较，杞菊组与补青颗粒高、中、低剂量组细胞增殖活力均降低（P<0.01）；与杞菊组比较，补青颗粒中剂量组细胞增殖活力明显降低（P<0.01）；补青颗粒不同剂量组间比较，补青颗粒中剂量组细胞增殖活力显著降低（P<0.01）。细胞免疫荧光结果显示Pink-1，Parkin，p62蛋白主要在细胞胞浆表达，细胞核内亦有少量表达。Western blot结果显示与空白组比较，模型组Pink-1，Parkin表达增加（P<0.01），p62表达降低（P<0.01）；与模型组比较，杞菊组与补青颗粒中剂量组Pink-1，Parkin表达增加（P<0.01），p62表达降低（P<0.01）；与杞菊组比较，补青颗粒中剂量组Pink-1，Parkin表达增加（P<0.01，P<0.05），p62表达降低（P<0.01）。结论：补青颗粒对高糖诱导的HLE细胞线粒体自噬相关蛋白的表达有显著影响，线粒体自噬的改变可能是补青颗粒改善白内障发病的机制之一。

Abstract

Objective: To discuss the effect of Buqing granules-medicated sera on the human lens epithelial cells induced by high glucose. Method: Human lens epithelial (HLE) cell lines were cultured in vitro. HLE cells of blank control group were cultured in the normal nutrient solution for 48 h. In addition to that

model group was also added with 30.0 mmol·L-1 glucose for 48 h. In addition to that

treatment group was also added with different medicated sera and cultured for 48 h. The cell's activity of proliferation was detected by CCK-8 assay. Protein expressions of Pink-1

Parkin

p62 were detected by Western blot. Result: According to CCK-8 assay

model group showed a higher HLE cells proliferation activity than the blank group (P<0.01). Compared with model group

the cell's activity of proliferation of Qiju group and high

middle and low-dose Buqing granules groups were lower (P<0.01). Compared with Qiju group

the cell's activity of proliferation of middle-dose Buqing granules group was obviously decreased (P<0.01). Among different Buqing granules groups

the cell's activity of proliferation of middle-dose Buqing Granules group was significantly decreased (P<0.01). According to immunofluorescence staining

Pink-1

Parkin

p62 proteins were expressed mainly in cell cytoplasm and rarely in nuclei. According to Western blot

compared with blank group

protein expression levels of Pink-1

Parkin of model group were increased (P<0.01)

and p62 expression was decreased (P<0.01). Compared with model group

protein expression levels of Pink-1

Parkin of Qiju group and middle-dose Buqing granules group were increased(P<0.01)

and p62 expression was decreased (P<0.01). Compared with Qiju group

protein expression levels of Pink-1

Parkin of middle-dose Buqing granules group were increased (P<0.01)

and p62 expression was decreased (P<0.01). Conclusion: Buqing granules have a significant effect on protein expressions related to mitochondrial autophagy of HLE cells induced by high glucose. The change in mitochondrial autophagy may be one of the mechanisms of Buqing Granules in alleviating cataract.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

补肾活血方调节PINK1/Parkin通路对卵巢早衰模型大鼠的影响

加味升降散调节PINK1/Parkin信号通路激活糖尿病肾病线粒体自噬的机制

线粒体自噬在肾纤维化中的作用机制及中医药干预研究进展

真武汤含药血清对心肌肥大细胞凋亡和线粒体自噬的影响

大黄黄连泻心汤加味对肥胖2型糖尿病大鼠内脏脂肪线粒体自噬及棕色化影响