不同商品规格巴戟天炮制品中游离蒽醌类成分含量的比较

陈红; 张清华; 陈鸣; 程再兴

doi:10.13422/j.cnki.syfjx.2017150005

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不同商品规格巴戟天炮制品中游离蒽醌类成分含量的比较

药剂与炮制 | 更新时间：2024-01-04

- 不同商品规格巴戟天炮制品中游离蒽醌类成分含量的比较
- Comparison of Contents of Five Free Anthraquinones in Processed Products of Morindae Officinalis Radix with Different Commercial Grades
- 中国实验方剂学杂志 2017年23卷第15期页码：5-9
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年基金项目（81001636）;福建省高等学校新世纪优秀人才项目;福建省自然科学基金项目（2016J01774）;校管-重点学科专项（X2014145-学科）;福建省中药炮制技术传承基地平台项目
- DOI：10.13422/j.cnki.syfjx.2017150005
  中图分类号： R283
- 纸质出版日期：2017
- 稿件说明：
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陈红, 张清华, 陈鸣, 等. 不同商品规格巴戟天炮制品中游离蒽醌类成分含量的比较[J]. 中国实验方剂学杂志, 2017,23(15):5-9.

CHEN Hong, ZHANG Qing-hua, CHEN Ming, et al. Comparison of Contents of Five Free Anthraquinones in Processed Products of Morindae Officinalis Radix with Different Commercial Grades[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 5-9.
陈红, 张清华, 陈鸣, 等. 不同商品规格巴戟天炮制品中游离蒽醌类成分含量的比较[J]. 中国实验方剂学杂志, 2017,23(15):5-9. DOI： 10.13422/j.cnki.syfjx.2017150005.

CHEN Hong, ZHANG Qing-hua, CHEN Ming, et al. Comparison of Contents of Five Free Anthraquinones in Processed Products of Morindae Officinalis Radix with Different Commercial Grades[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 5-9. DOI： 10.13422/j.cnki.syfjx.2017150005.

摘要

目的：以巴戟天中5种游离蒽醌类化合物（2-羟基-3-甲基蒽醌，2-羟基-1-甲氧基蒽醌，1，8-二羟基蒽醌，甲基异茜草素-1-甲醚，甲基异茜草素）为指标，对市场上流通的不同商品规格的巴戟天饮片的质量进行检测。方法：三氯甲烷提取后采用HPLC同时检测上述5种成分的含量，Phenomenex-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈（A）-0.4%磷酸（B）梯度洗脱（0~15 min，15%~30%A；15~70 min，30%~45%A；70~90 min，45%~65%A；90~100 min，65%~90%A），流速1 mL·min-1，柱温30 ℃，检测波长277 nm，进样量10 μL。结果：上述5种游离型蒽醌分别在0.010~0.250，0.060~1.500，0.004~0.100，0.050~1.250，0.025~0.625 μg与其峰面积呈良好线性关系，r分别为0.999 7，0.999 1，0.999 6，0.999 7，0.999 5。加样回收率分别为98.2%，99.6%，97.9%，98.5%和97.7%，RSD分别为1.4%，1.6%，2.2%，1.6%和1.7%。结论：所建立的方法准确、可靠，能同时检测上述5种游离蒽醌类成分的含量，适用于巴戟天饮片的质量评价。

Abstract

Objective: To establish a sensitive and specific high performance liquid chromatography method (HPLC) for simultaneous determination of five free anthraquinones (2-hydroxyl-3-methyl anthraquinone

2-hydroxy-1-methoxy anthraquinone

8-dihydroxy anthraquinone

rubiadin-1-methyl ether and rubiadin) in Morindae Officinalis Radix. Method: The analytic procedure was carried out on a Phenomenex C18 column(4.6 mm×250 mm

5 μm) and detected with an UV detector at the wavelength of 277 nm.Gradient elution was carried out with 0.4% phosphoric acid and acetonitrile at the flow rate of 1.0 mL·min-1. The column temperature was set at 30 ℃. Result: The calibration curves were linear in the range of 0.010-0.250 μg for 2-hydroxyl-3-methyl anthraquinone with r of 0.999 7

0.060-1.500 μg for 2-hydroxy-1-methoxy anthraquinone with r of 0.999 1

0.004-0.100 μg for 1

8-dihydroxy anthraquinone with r of 0.999 6

0.050-1.250 μg for rubiadin-1-methyl ether with r of 0.999 7 and 0.025-0.625 μg for rubiadin with r of 0.999 5

respectively.The average recoveries of these five components were 98.2%

99.6%

97.9%

98.5% and 97.7%

respectively.Relative standard deviations were 1.4%

1.6%

2.2%

1.6% and 1.7%

respectively. Conclusion: The established method is accurate and reliable

and it can be used for the simultaneous determination of these five components

which provides a scientific basis for the quality evaluation of Morindae Officinalis Radix.

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