Objective: To compare the difference of in situ small intestinal absorption in rats of notoginsenoside R1

ginsenoside Rg1 and ginsenoside Rb1 in original powder

ultra-fine powder and cell wall-broken powder of Notoginseng Radix et Rhizoma. Method: In situ perfusion method in rats was adopted

the concentration of notoginsenoside R1

ginsenoside Rg1

ginsenoside Rb1 and phenol sulfonphthalein in intestinal circulating perfusate were determined simultaneously by HPLC

which is used to study on the absorption of notoginsenoside R1

ginsenoside Rg1and ginsenoside Rb1 from Notoginseng Radix et Rhizoma samples in the small intestine of rats. Result: The absorption rate constants (Ka) of notoginsenoside R1 in the original powder

ultra-fine powder and cell wall-broken powder were 0.049 80

0.037 37

0.034 60 h-1

Ka of ginsenoside Rg1 were 0.044 83

0.032 48

0.036 50 h-1

Ka of ginsenoside Rb1 were 0.078 40

0.095 48

0.084 78 h-1

respectively.The absorption percentages (P) of notoginsenoside R1 were 9.543%

7.662%

5.858%

those of ginsenoside Rg1 were 8.602%

7.154%

6.667%

those of ginsenoside Rb1 were 14.60%

17.86% and 15.64%

respectively. Conclusion: There are differences in the absorption amount and rate of notoginsenoside R1

ginsenoside Rg1 and ginsenoside Rb1 in three kinds of Notoginseng Radix et Rhizoma powder

but the absorption of ultra-fine powder and cell wall-broken powder has no significant superiority by comparing with the original powder

selection of original powder as raw material is better.