Objective: To improve the quality standard of Psoraleae Fructus

solve the problem of lacking relevant reference substance

and establish a content determination method for 7 compounds in Psoraleae Fructus decoration piece based on the standard reference extract. Method: HPLC was performed on Thermo-C18 endcapped column (4.6 mm×250 mm

5 μm)

with water-methanol-acetonitrile system as the mobile phase for gradient elution. The column temperature was 30 ℃; the injection volume was 10 μL

and the detection wavelength was set at 254 nm. Self-prepared reference extracts of Psoraleae Fructus was used for determining the contents of psorale

isopsorale

psoralidin

corylifolinin

bavachinin and bakuchiol; then the contents of the 7 components in 12 batches of Psoraleae Fructus decoration piece were determined and compared with the experimental results obtained with the reference substance determination method. Result: The seven markers including psorale

isopsorale

psoralidin

corylifolinin

bavachinin and bakuchiol

reached baseline separation and showed good linearity. Their average recovery rate was 97.4%

98.8%

99.6%

102%

99.6%

98.0%

103% and RSDs of seven compounds were all no more than 2.8%. The reference extraction determination method showed high precision

stability and reproducibility

and there was no significant difference with the results of reference substance determination method. Conclusion: The reference extraction determination method was simple

and can be used for the contents determination of 7 compounds in Psoraleae Fructus decoration piece.