木瓜苦桑片对Ⅱ型糖尿病大鼠的影响

高玲; 许立拔; 王巍; 林彩霞; 杨沛霖; 巫玲玲; 庞晓军; 蒋伟哲

doi:10.13422/j.cnki.syfjx.2017150166

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木瓜苦桑片对Ⅱ型糖尿病大鼠的影响

药理 | 更新时间：2024-01-04

- 木瓜苦桑片对Ⅱ型糖尿病大鼠的影响
- Effect of Mugua Kusang Tablets on Type 2 Diabetes Rats
- 中国实验方剂学杂志 2017年23卷第15期页码：166-172
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西科技计划项目（桂科AB16380187）
- DOI：10.13422/j.cnki.syfjx.2017150166
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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高玲, 许立拔, 王巍, 等. 木瓜苦桑片对Ⅱ型糖尿病大鼠的影响[J]. 中国实验方剂学杂志, 2017,23(15):166-172.

GAO Ling, XU Li-ba, WANG Wei, et al. Effect of Mugua Kusang Tablets on Type 2 Diabetes Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 166-172.
高玲, 许立拔, 王巍, 等. 木瓜苦桑片对Ⅱ型糖尿病大鼠的影响[J]. 中国实验方剂学杂志, 2017,23(15):166-172. DOI： 10.13422/j.cnki.syfjx.2017150166.

GAO Ling, XU Li-ba, WANG Wei, et al. Effect of Mugua Kusang Tablets on Type 2 Diabetes Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(15): 166-172. DOI： 10.13422/j.cnki.syfjx.2017150166.

摘要

目的：研究木瓜苦桑片对高脂高糖联合低剂量链脲佐菌素诱导的Ⅱ型糖尿病大鼠血糖、血脂、肝肾功能和胰腺组织的影响。方法：雄性SD大鼠给予高脂饲料喂养4周后，链脲佐菌素（45 mg·kg-1）给药1次，建立糖尿病大鼠模型，将造模成功的大鼠随机分为模型组，二甲双胍组（285 mg·kg-1），木瓜苦桑片高、中、低剂量组（2 600，1 300，650 mg·kg-1），另设正常组，每组10只，每天灌胃给药1次，连续4周，记录每只大鼠24 h的尿量、饮水量、摄食量，分别测定血糖值（FBG），胰岛素（INS），过氧化氢酶（CAT），超氧化物歧化酶（SOD），丙二醛（MDA），一氧化氮（NO），血清总胆固醇（TC），甘油三酯（TG），低密度脂蛋白胆固醇（LDL-C），高密度脂蛋白胆固醇（HDL-C），丙氨酸氨基转移酶（AST），天冬氨酸氨基转移酶（ALT），肌酸激酶（CK），肌酐（Cr）和尿素氮（BUN）水平，取胰腺苏木素-伊红（HE）染色观察组织结构。结果：与正常组比较，模型组的大鼠摄食量，饮水量，尿量，FBG，INS，MDA，NO，LDL-C，TC，TG，AST，ALT，BUN，Cr，CK水平均明显升高，CAT，SOD，HDL-C水平降低（P<0.05，P<0.01）；与模型组比较，各给药组大鼠摄食量，饮水量，尿量，FBG，INS，MDA，NO，LDL-C，TC，TG，AST，ALT，BUN，Cr，CK水平均明显降低，CAT，SOD，HDLC水平升高（P<0.05，P<0.01）；与正常组比较，模型组大鼠胰岛细胞数目减少，排列紊乱，颜色深，细胞间质和细胞间界限不清晰；与模型组比较，各给药组大鼠胰岛细胞数目增多，排列整齐，细胞形态恢复规则。结论：木瓜苦桑片有明显降血糖及血脂作用，可增强机体抗氧化损伤的能力，对肝肾功能具有明显改善作用，可维持胰岛正常结构和功能。

Abstract

Objective: To investigate the effects of Mugua Kusang tablets on blood glucose

lipid

function of liver

kidney and pancreas issues in type 2 diabetes rats induced by high sucrose/high fat diet combined wih low dose streptozotocin(STZ). Method: Diabetes model was induced by feeding with high sucrose/high fat diet for 4 weeks and STZ at dose of 45 mg·kg-1 injection in male SD rats

then the successfully modeled rats were divided randomly into the model group

metformin group (285 mg·kg-1)

and Mugua Kusang tablets high dose

medium dose and low dose groups (2 600

1 300

650 mg·kg-1). Another 10 normal rats were assigned as the normal control group

n=10 in each group. Intragastric administration was given once a day for 4 weeks

and 24 h urine volume

water intake

and food intake were recorded for all rats. Then the levels of fasting blood glucose(FBG)

insulin(INS)

catalase(CAT)

superoxide dismutase(SOD)

methane dicarboxylic aldehyde(MDA)

nitric oxide(NO)

serum total cholesterol(TC)

triglyceride(TG)

low density lipoprotein cholesterol(LDL-C)

high-density lipoprotein cholesterol(HDL-C)

alanine aminotransferase(AST)

aspartate transaminase(ALT)

creatine kinase(CK)

creatinine(Cr) and urea nitrogen(BUN) were measured

and pancreatic structural change was observed by htoxylin-eosin(HE) staining. Result: As compared to the normal control group

the levels of food-intake

water-intake

urine volume

FBG

INS

MDA

LDL-C

AST

ALT

BUN

CK and Cr were increased

while levels of CAT

SOD and HDL-C were decreased in the model group (P<0.05

P<0.01). As compared to the model group

the levels of food-intake

water-intake

urine volume

FBG

INS

MDA

LDL-C

AST

ALT

BUN

CK and Cr were decreased

while the levels of CAT

SOD and HDL-C were increased in all treatment groups (P<0.05

P<0.01). As compared to the normal control group

islet cells count was decreased

with a disordered arrangement and darker color in model group

and the boundaries were indistinct between the intercellular substance and cells. As compared to the model group

the islet cells count was increased in ordered arrangement and regular forms and structures in various treatment groups. Conclusion: Mugua Kusang tablets can significantly decrease blood glucose and serum lipid

enhance the body's antioxidant injury ability

significantly improve the hepatorenal function

and maintain the normal structure and function of the islet.

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