Objective: This study is aimed to clone the key enzyme 1-deoxylated xylose-5-phosphate synthase (DXS) genes and analyze its tissue-specific expression in Atractylodes lancea. Method: The full-length cDNA sequence was cloned from the DXS gene fragment based on transcriptome sequencing data of A. lancea ge nerated in our previous study. The expression levels of DXS gene in different tissues of A. lancea were detected by Real-time PCR

with tublin as the reference gene. Result: The full-length cDNA sequence of DXS gene is 2 151 bp

encoding 716 amino acids

which has been deposited in GenBank (accession number KY659208). Results of phylogenetic analysis showed that the sequence had high homology with DXS gene of other compositae plants

such as Stevia rebaudiana. Real-time PCR analysis showed that the DXS gene was mostly expressed in leaves

followed by flowers

rhizome and roots. Conclusion: Full-length cDNA sequence of DXS gene in A. lancea has been obtained for preliminary bioinformatics analysis

revealing differential expression in different tissues. The results will provide a groundwork for studying the function of DXS in terpenoid biosynthesis of A. lancea.