Objective: To study on chemical constituents from the seeds of Celastrus angulatus by means of modern separation method and spectrum technology

and provide experimental data for its research

development and utilization. Method: Dry seeds of C. angulatus were extracted by petroleum ether for 3 times. Its extracts were then further extracted by 90% methanol solution for 3 times. From the methanol layer

the compounds were isolated by silica gel column chromatography and petroleum ether-ethyl acetate solvent system (10:1-2:8) was used for gradient elution. The eluent received decompressed drying and was further separated and purified by preparative HPLC with methanol-H2O solvent system

and finally purified by recrystallization. The compounds were identified on the basis of physic-chemical constants and spectral analysis. Result: Six compounds were obtained and identified as 1β

15-diacetoxy-8β

9β-dibenzoyloxy-β-dihydroagarofuran (1)

angulatueoid H (2)

angulatinoid Ⅱ (3)

angulatinoid Ⅲ (4)

1β

15-diacetoxy-8α-hydroxy-9β-benzoyloxy-β-dihydroagarofuran (5) and 1β

2β

8α-triacetoxy-9β-benzoyloxy-β-15-nicotinoyloxy-β-dihydroagarofuran (6) respectively

with their NMR data detected through 1D (1H

13C-NMR and DEPT) and 2D NMR (1H-1H COSY

HSQC

HMBC and NOESY) techniques. Conclusion: Compounds 1

3 and 5 were obtained from C. angulatus for the first time. Compound 4 was obtained from the nature for the first time.