Objective: To study on the chemical compositions of medium polarity part in 70% ethanol extract of Millettia nitita var.hirsutissima. Method: Silica gel column

Sephadex LH-20 column

reverse phase chromatography technology and preparative chromatography were used for separation and purification

and their chemical structures were identified according to the nuclear magnetic resonance spectrum results. Result: The 15 compounds were isolated from 70% ethanol extract by chromatography and their structures were identified as formononetin (1)

8-methylretusin (2)

astragaluquinone (3)

salicylic acid (4)

calycosin(5)

genistein (6)

wistin (7)

3'

4'

7-trihydroxy flavone (8)

butein(9)

sissotrin(10)

mncodianin F (11)

ononin (12)

vitexin (13)

4'

8-dimethoxyl-7-O-β-D-glucopyranosyl isoflavone (14)

and (2S)-1-O-heptatriacontanoyl glycerol (15). Conclusion: Compounds 2 and 11 were isolated from this plant for the first time; compounds 3

8

9

13 and 14 were isolated from this genus for the first time.