Objective: To study the chemical constituents and analgesic activity of Primula denticulata ssp. sinodenticulata and to lay the foundation for the development of Primula plants in the future. Method: The major compounds from 85%ethanol extract of P. denticulata ssp. sinodenticulata were isolated by solvent extraction

recrystallization method

as well as various column chromatography methods including silica gel

RP-18

and Sephadex LH-20 column chromatography. The structures of the compounds were identified by spectral technique combined with TLC method

and their analgesic activity of samples and selected compounds from P. denticulata ssp. sinodenticulata was evaluated by using acetic acid induced writhing method and hot plate method. Result: Seven monomeric compounds were obtained from ethyl acetate and n-butanol fractions and identified as 2

2'-dihydroxychalcone (1)

5-hydroxyflavone (2)

3'-hydroxyflavone (3)

quercetin 3-O-(2

6-di-O-β-D-glucopyranosyl)-β-D-glucopyranoside (4)

riccardin C (5)

primeverin (6)

and hexabecanoic acid (7). All of the compounds were obtained from this plant for the first time. Compounds 2

3

and 7 could reduce the times of writhing in mice induced by acetic acid

but could not increase the pain threshold of mice induced by hot plate. Conclusion: P. denticulata ssp. sinodenticulata was rich in flavonoids and phenolic compounds. Some of the flavonoids had peripheral analgesic activity

and the analgesic effect was similar to or even better than that of aspirin.