Objective: To investigate the antidiabetic effect of Acanthopanax senticosus total glycosides extract based on GC-MS metabonomics and to explore its mechanism. Method: Diabetic mice model was established by intraperitoneal injection of streptozotocin and alloxan

multivariate statistical analysis was employed to analyze serum metabolites

NIST 11 database and authentic standards were used to analyze and identify differential metabolites

and MetPA was employed to analyze the corresponding metabolic pathways. Result: The diabetic mice model was duplicated successfully.After administration of A.senticosus total glycosides extract

the levels of metabolites in the model group were detected

hexadecanoic acid

octadecanoic acid

linoleic acid

tyrosine

phenylalanine

aspartic acid and serine showed decrease;alanine

lactic acid

citric acid

glutamine

valine

iso(leucine)

β-hydroxybutyric acid and glycine showed increase

which indicated a tendency towards normality.Pathway enrichment analysis by MetPA displayed that these metabolites were mainly involved in six metabolic pathways which therefore were closely related to diabetes. Conclusion: A.senticosus total glycosides extract displays a significant hypoglycemic effect

which may be achieved by regulation of energy metabolism

amino acid metabolism

amino acid biosynthesis and organic acid metabolism.