Objective: To predict and investigate the molecular mechanism of active principle region of Yangxing Tongmai formula (apr-YTF) in inducing bone marrow mesenchymal stem cells (BMSCs) myocardial differentiation based on miRNA level. Method: BMSCs from acute myocardial infarction (AMI) rats of blood-stasis model were extracted and cultivated. Then apr-YTF serum was used as experimental group and negative control serum was used as control group

respectively. Total RNAs were extracted and differently expressed miRNAs were detected by expression profile chip for hierarchical clustering analysis. Bioinformatics method was used to predict target genes and analyze the functions of differential miRNAs regulation. Result: There were a total of 26 differently expressed miRNAs

8 were up-regulated

and 18 were down-regulated. In screening and predicting

100 target genes were involved in 19 kinds of biological processes

11 kinds of cellular components

and 10 kinds of molecular functions. Axon guidance

Transcriptional misregulation in cancer

and AMPK signaling pathway were enriched. Rno-miR-93-5p

rno-miR-204-5p

rno-miR-128-3p were the center of the molecular network

and rno-miR-204-5p was down-regulated significantly. Conclusion: The results showed that rno-miR-204-5p

rno-miR-93-5p and Axon guidance pathway may play a role in the differentiation process of BMSCs into cardiomyocytes induced by apr-YTF.