Objective: To investigate the efficacy and compatibility mechanism of Si Junzitang. Method: Chemical components

targets and diseases related to the four traditional Chinese medicine (TCM) herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Compound-target network

target-disease network

protein-protein interaction(PPI) network

and target-pathway network were constructed to explore the mechanism of Si Junzitang. Result: Totally 27 components

93 targets and 215 diseases related to the four TCM herbs were obtained through screening. The PPI network contains 68 targets

in which key targets involving FOS

ESR1

AR

PPARG and so on. There were 121 gene ontology(GO) entries

including 96 biological process entries

13 molecular function entries and 12 cellar component entries. Besides

there were 3 KEGG pathways

involving Neuroactive ligand-receptor interaction

calcium signaling pathway and cancer pathway. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Si Junzitang

in order to lay a solid foundation for further studies on the mechanism of action.