Objective: To investigate the efficacy and compatibility mechanism of Wuzhuyu Tang. Method: The chemical components and targets related to the four Chinese herbal medicines were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The compound-target network

protein-protein interaction(PPI) network

and target-pathway network were constructed to explore the mechanism of Wuzhuyu Tang. Result: The compound-target network contain 29 chemicalcomponents and 77 targets. The hub targets involve PTGS2

PTGS1 and so on.The PPI network contain 70 proteins

in which the hub proteins involve AP-1

BCL2

HSP90α and so on. In the gene ontology(GO) functional enrichment analysis

there are 145 GO terms (100 Biological Process

23 Molecular Function

22 Cellar Component). The pathway enrichment analysis contains 3 pathways:calcium signaling pathway

neuroactive ligand-receptor interaction

and pathways in cancer. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Wuzhuyu Tang

thuslaying a solid foundation for deeply study on the mechanism of action.